Surena Vahabi
Shahid Behesti University of Medical Sciences, Iran
Title: Neurological effects of Cyclosporine and Phenytoin in dentistry, Bio gene marker expression in gingival hyperplasia
Biography
Biography: Surena Vahabi
Abstract
Background: Epilepsy is a major concern in both developed and under developing countries and one of the oral manifestations of Epilepsy is gingival overgrowth. Since the cellular mechanisms behind the gingival overgrowth has remained unknown especially in pediatrics, the aim of this study was to investigate the effect of CSA and PHT on the bio gene marker expression and compare it among adults and pediatrics.
Methods: Gingival fibroblasts which had been harvested from adults and pediatrics with normal gingiva were incubated with CSA and PHT, and then cultured for 48 hours. MMP (1 , 2), TIMP, Col , Eln, Lysyl, Cat L, B and mRNA level in culture were determined by reverse transcription polymerase chain reaction (RT-PCR), amount of TGFB and EGF were assessed by enzyme-linked immunosorbent assay(ELISA). The alpha error level was set at 0.05 for statistical significance.
Results: CSA and PHT stimulated the TGFB and CATB productions and inhibited expression of MMP1 by fibroblasts. CSA suppressed TIMP in pediatrics but PHT stimulated its expression. In adults, both CSA and PHT increased TGFB, Lysyl and EGF level. CSA reduced Eln level whereas PHT stimulated its level. PHT increased the CAT B level when CSA inhibited TIMP expression
Discussion: It seems that in pediatrics MMP1/TIMP system and in adults Lysyl/Eln pathway play an important role in impaired collagen metabolism.